Non-radioactive screening of DNA polymerases by using oligo r(A-U) as primer/template for reverse transcriptase activity.
نویسندگان
چکیده
polymerase (lane e), and Thermococcus litoralis-derived Vent@ DNA polymerase (lane f) synthesized poly d(A-T), while Pyrococcus sp・-derived PyrobestTM DNA polymerase (lane g) failed to synthesize poly d(A-T). ThoughBcaBESTTM DNA polymerase was known to have RT activity (including Takara's RT-PCR kit), the RT activities of KOD and Vent@ DNA polymerases have not been yet reported. It should be mentioned that some thermostable DNA polymerases, such
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ورودعنوان ژورنال:
- Japanese journal of infectious diseases
دوره 52 5 شماره
صفحات -
تاریخ انتشار 1999